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NOTCH 1 Antibody

Rabbit Polyclonal
NCI Collaboration
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Immunohistochemistry of Rabbit anti-Notch1 antibody.  
Tissue:  Exocrine glands of human pancreas
Fixation: FFPE 
Primary antibody:  Notch1 antibody at 1:200 
Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.
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Number:
100-401-407
Size:
200 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

NOTCH 1 Antibody Properties

Description
Anti-NOTCH 1 (Cleaved N terminal) (Human specific) (RABBIT) Antibody - 100-401-407
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:60,000
Western Blot : 1:500- 1:2,000
Immunohistochemistry: 1:200
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
90 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
GeneName
NOTCH1
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NOTCH 1 Antibody Description

Background
Anti-Notch 1 Antibody recognizes Notch 1 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Synonyms
Neurogenic locus Notch homolog protein 1, hN1, Translocation-associated Notch protein TAN-1.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Notch 1 is reactive with Notch 1 by western blotting, Immunohistochemistry (IHC) and ELISA.    An 80 kDa band corresponding to Notch 1 was observed at a 1:500 dilution.  Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This antiserum is directed against human NOTCH 1.  Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology).  This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells.  Only the cleaved intracellular (activated) form (NICD) is detected.  No reactivity is detected against mouse N2, N3 or N4.  The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW (2000) Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells. Leukemia 12: 2095-2102. Gridley T (2003)  Notch signaling and inherited disease syndromes. Hum. Mol. Genet. 12: Suppl 1, R9-R13.
Specific Reference
Gupta S, Li S, Abedin Md J, Wang L, Schneider E, Najafian B, Rosenberg M. Effect of Notch activation on the regenerative response to acute renal failure. Am J Physiol Renal Physiol 298: F209–F215, 2010. Published online before print October 14, 2009, doi: 10.?1152/?ajprenal.?00451.?2009 Solomon A, Mian Y, Ortega-Cava C, et al. Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants. Dev. Biol. 2008 Apr 15;316(2):191–199. Harrison H, Simões BM, Rogerson L, Howell SJ, Landberg G, and Clarke RB. (2013) Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling. Breast Cancer Research 2013, 15:R21 doi:10.1186/bcr3396. Stylianou S, Clarke RB, Brennan K. Aberrant activation of notch signaling in human breast cancer. Cancer Res. 2006 Feb 1;66(3):1517–1525.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-405

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, dog, chimpanzee
Catalog Number
100-401-406

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-408

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
Human, mouse
Catalog Number
200-401-698

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry