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Primary Antibodies  >  Stem Cell Antibodies
 

Angiopoietin 2 Antibody

Rabbit Polyclonal
Code

100-401-402
Size

200 µL
Price

$309.00
Availability

Ships next business day.
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Immunocytochemistry of anti-ANG2 Antibody in PC12 cells
Tissue: Human prostate cancer cell line (PC12)
Fixation: 5 min in 100% methanol
Ag Retrieval/permeabilization:  1hr 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween 
Primary antibody: 1:200 dilution overnight at +4°C 
Secondary Ab: 488 Gt anti rabbit 1:1000 1 hr RT
Staining: Ang2 stained yellow/green, plasma membranes stained red, cell nuclei stained blue with DAPI (1.43 µM)
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: AGPT 2 antibody, Agpt2 antibody, ANG 2 antibody, ANG2 antibody, Angiopoietin 2a antibody, Angiopoietin 2B antibody, Angiopoietin2 antibody, ANGPT 2 antibody, ANGPT2 antibody, Tie2 ligand antibody

Angiopoietin 2 Antibody Properties

Description
Anti-Angiopoietin 2 (RABBIT) Antibody - 100-401-402
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
human, mouse
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 1:200-1:500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
GeneName
ANG-2
Download CofA
Lot#  

Angiopoietin 2 Antibody Description

Background
Anti Antiopoietin-2 Antibody recognizes Angiopoietin-2 (Ang-2) that has importance in development of the endothelium through regulation of tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding and thus blocks receptor phosphorylation.  Ang-2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus. This antibody is suitable for cardiovascular research.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Angiopoietin 2 Antibody is suitable for western blotting and other antibody based assays.  A 1:500 dilution is recommended for western blotting.  The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum.  This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean, strong signal.
Purity/Specifity
Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins.  A 75 kDa band is detected when assayed by immunoblot.  This reagent cross-reacts with human angiopoietin-2.  Partially cross-reactivity is noted with human angiopoietin-1.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Suri, C., Jones, P.F., Patan, S., Bartunkova, S., Maisonpierre, P.C., Davis, S., Sato, T.N. and Yancopoulos G.D. (1996) Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. Cell, 87(7): 1171-1180. Davis, S., Aldrich, T.H., Jones, P.F., Acheson, A., Compton, D.L., Jain, V., Ryan, T.E., Bruno, J., Radziejewski, C., Maisonpierre, P.C. and Yancopoulos, G.D. (1996)  Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning.  Cell 87(7): 1161-1169. Maisonpierre, P.C., Suri, C., Jones, P.F., Bartunkova, S., Wiegand, S.J., Radziejewski, C., Compton, D., McClain, J., Aldrich, T.H., Papadopoulos, N., Daly, T.J., Davis, S., Sato, T.N. and Yancopoulos, G.D.  (1997)  Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science 277(5322): 55-60.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-000-402

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse
Catalog Number
100-401-403

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-4165N

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry