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Primary Antibodies  >  Infectious Disease Antibodies
 

Neuraminidase Antibody

Rabbit Polyclonal
Code

100-401-396
Size

200 µL
Price

$309.00
Availability

Ships next business day.
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Western blot analysis using Rockland Immuno-chemical's anti-Neu1 antibody to detect neuraminidase in cell lysates (5 µg each) of fibroblasts from patients. Anti-Neu1 (top) and anti-PPCA antibodies (bottom) were used to analyze western blots containing protein (5 µg) from fibroblast lysates of a healthy person (WT), five patients with type I sialidosis and five patients with type II sialidosis.  This antibody detects 46 kDa neuraminidase.  Communicated with permission by E. Bonten.  See Bonten et al (2000) for additional details.
Immunocytochemical localization using Rockland Immunochemical's anti-Neu1 antibody to detect neuraminidase in cell lysates of fibroblasts from a healthy person (WT and patient 7 (see above).  Anti-Neu1 antibodies were used in conjunction with FITC-conjugated Gt-a-Rabbit IgG (code # 611-102-122). The nuclei were stained with DAPI. The magnification is 400x.  Communicated with permission by E. Bonten.  See Bonten et al (2000) for additional details.
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Synonyms: Acetylneuraminyl hydrolase antibody, G9 sialidase antibody, Lysosomal sialidase antibody, N acetyl alpha neuraminidase 1 antibody, NANH antibody, Neu 1 antibody, Neu1 antibody, SIAL1 antibody, Sialidase 1 antibody

Neuraminidase Antibody Properties

Description
Anti-Neuraminidase (Neu1) (RABBIT) Antibody - 100-401-396
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
IF Microscopy : 1:500
Western Blot : 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
GeneName
NEU1
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Lot#  

Neuraminidase Antibody Description

Background
Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of a-glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates. They are widely spread in nature, occurring in viruses, bacteria, fungi, protozoa, birds and mammals. Together, the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs. Three types of neuraminidase are found in mammals and are defined as lysosomal, plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution. Lysosomal N-acetyl-a-neuraminidase (NEU1) has significant primary structure characteristics of other mammalian and microbial sialidases with similar substrate specificity. However, unlike other members of this family, lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation. The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight, multi-protein complex containing PPCA, ß-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase. The autosomal recessive genetic deficiency of NEU1 is associated with sialidosis, a neurodegenerative lysosomal storage disorder.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a bacterially expressed and purified fusion protein excluding the signal peptide (first 45 amino acids) and the first 6 N-terminal amino acids of the processed form of human lysosomal neuraminidase.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antibody is suitable for western blotting, immunocytochemistry, immunoprecipitation, transfected cell culture, primary cell culture, and ELISA. This product was assayed on immunoblots against human primary fibroblast cell lysates. A single band of the expected apparent molecular weight was observed at a 1:500 dilution incubated for 2 h at room temperature. For immunofluorescence microscopy a 1:500 dilution is recommended incubated for 2 h at room temperature or alternatively, a 1:1,000-1:5,000 dilution can be used incubated overnight at room temperature. Staining is best on paraformaldehyde-fixed cultures. The optimal dilution and time of incubation is dependent on the methods used, the type of cells or tissue, and the amount of protein present. Neuraminidase is not very abundant in most tissues and its detection with the antibodies may require further optimization. Researchers should determine optimal titers for other applications.
Purity/Specifity
This antiserum is directed against Neu1 and reacts with the Neu1 from human tissues.  Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Bonten, E. J.; Arts, W. F.; Beck, M.; Covanis, A.; Donati, M. A.; Parini, R.; Zammarchi., E.; d'Azzo, A. (2000) Novel mutations in lysosomal neuraminidase identify functional domains and determine clinical severity in sialidosis. Hum. Molec. Genet. 9: 2715-2725. de Geest, N.; Bonten, E.; Mann, L.; de Sousa-Hitzler, J.; Hahn, C.; d'Azzo, A. (2002)  Systemic and neurologic abnormalities distinguish the lysosomal disorders sialidosis and galactosialidosis in mice. Hum. Molec. Genet. 11: 1455-1464. Bonten EJ, d'Azzo A.(2000) Lysosomal neuraminidase. Catalytic activation in insect cells is controlled by the protective protein/cathepsin A. J Biol Chem. Dec 1;275(48):37657-63.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
100-401-396

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
200-4136

Product Type
GFP and Epitopes;
Applications
WB, IF
Catalog Number
200-4236

Product Type
GFP and Epitopes;
Applications
FLISA, WB, IF
Catalog Number
200-430-036

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry