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Primary Antibodies  >  Transcription Factor Antibodies
 

CIITA Antibody

Rabbit Polyclonal
Code

100-401-249
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Western blot of Anti-CIITA (1-333) antibody, generated by immunization with bacterially produced FLAG-CIITA aa 1-333, was tested by western blot against lysates of Cos-7 cells after transient transfection, separately, with pcDNA3-FLAG-CIITA-336-884 and pcDNA3-HA-CIITA.  For transfection, Fugene 6 (Roche) was used according to the manufacturer's instructions for a 6-well plate format.  Cells were lysed 24 h post-transfection in 200 uL of 1x SDS-sample buffer, heated at 96C for 5', and vortexed for 30 sec.  Samples (10 uL each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45' using TTBS supplemented with 5% non-fat dry milk.  All incubations were performed at room temperature.  In panel A, both samples on PVDF were incubated with 10 ug/mL mouse anti-FLAG antibody (Sigma) for 45'.  After 5X washes with TTBS, reaction with ALP rabbit anti-mouse IgG at 200 ng/mL proceeded for 45' following again by washing as before.  The blot was developed using BCIP/NBT.  This blot demonstrates that FLAG-CIITA-336-842 was successfully over-expressed in the Cos-7 cells.  In panel B, both samples on PVDF were incubated with a 1:500 dilution of Rockland's anti-CIITA (1-333) for 45'.   After 5X washes with TTBS, reaction with HRP goat anti-rabbit IgG at 10 ng/mL proceeded for 45' following again by washing as before.  The membrane was covered with Pico West Substrate solution (Pierce) for 5' and was then placed between the two layers of a standard sheet protector.  Kodak O-MAT film was exposed to the blot for 30 sec and was immediately developed.  The lane containing the lysate of pcDNA3-HA-CIITA transfected cells contains a single band at ~130 kD molecular weight, whereas the lane containing lysate from pcDNA3-FLAG-CIITA-336-842 transfected cells shows no reactivity.  This blot demonstrates that anti-CIITA (1-333) is specific for amino acids 1-333 of CIITA and that the antibody is not cross reactive with the FLAG portion of the immunogen.
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Synonyms: MHC class II transactivator CIITA MHC2TA

CIITA Antibody Properties

Description
Anti-CIITA aa 1-333 (RABBIT) Antibody - 100-401-249
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:3,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
90 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CIITA, MHC2TA
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Lot#  

CIITA Antibody Description

Background
Anti-CIITA antibody detects CIITA. The transactivator CIITA regulates basal and interferon-induced expression of Major Histocompatibility Complex class II genes. CIITA restores expression of all MHC class II gene expression in mutant cells and corrects regulatory defects of MHC class II genes. Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression. Antigen can be obtained from Raji cell lysates. Typically levels of CIITA expression are too low to detect endogenous levels of protein expression. Transiently transfected cells are usually employed to study this transcription factor.
Immunogen
The immunogen used for this study was a bacterially produced recombinant FLAG-CIITA corresponding to amino acids 1 through 333 of the human protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
For immunoblotting a 1:500 dilution is recommended. Researchers should determine optimal titers for other applications.
Purity/Specifity
Anti-CIITA antibody was prepared from monospecific antiserum by delipidation and defibrination.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-192

Product Type
Primary Antibodies;
Applications
ELISA, WB, GS, Other
Reacts With
human, mouse, rat
Catalog Number
100-401-195

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-401-192

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-270

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry