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Primary Antibodies  >  Transcription Factor Antibodies
 

IKK beta Antibody

Rabbit Polyclonal
Code

100-401-220
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Western blot of anti-IKKß antibody reacted with HeLa cell extract.  All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.  The membrane was blocked in 5% dry milk for 2 h.  After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h.  Washes with buffer were performed 4 times for 5' each.  The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h.   Washes with TBS preceded color development.
Rockland's Anti-IKKß antibody was diluted 1:500 to detect IKKß in human placenta tissue.  Tissue was formalin fixed and paraffin embedded.  No pre-treatment of sample was required.  The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit beta I-kappa-B-kinase beta, IkBKB, IKK-beta, IKK-B EC=2.7.11.10 I-kappa-B kinase 2 IKK2 Nuclear factor NF-kappa-B inhibitor kinase beta NFKBIKB

IKK beta Antibody Properties

Description
Anti-IKKß (RABBIT) Antibody - 100-401-220
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:200 - 1:1,000
Immunochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
IKBKB, IKKB
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Lot#  

IKK beta Antibody Description

Background
NFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death.  In the cytoplasm NFkB is negatively modulated by the inhibitory proteins IkB.  In turn IkB is phosphorylated by a cellular kinase complex called IKK.  IKK is a heterodimer composed of two kinases: IKK-a and IKK-b that phosphorylate IkB leading to its degradation and the resulting translocation of NFkB to the nucleus.  IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF- a, endotoxins and overexpression of cellular kinases like MEKK1.  Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.
Immunogen
IKKb peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This product was assayed by immunoblot and found to be reactive against IKKb at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  Anti- IKKb is suitable for the detection by immunoblot of human, mouse and rat IKKb showing an 87 kDa band.  This product was assayed by immunoblot and found to be reactive against IKKb at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  Anti- IKKb is suitable for the detection by immunoblot of human, mouse and rat IKKb showing an 87 kDa band. 
Purity/Specifity
This product was prepared from monospecific antiserum by delipidation and defibrination.  Anti- IKKb may react non-specifically with other proteins.   Control peptide (code #100-401-220p) will compete only with the specific reaction of antiserum with the IKKb subunit.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
100-401-219

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
100-4167C

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
100-4186

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry