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Primary Antibodies  >  Kinase and Phosphatase Antibodies
 

Erk2 Antibody

Rabbit Polyclonal
Code

100-401-218
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's Affinity Purified anti-p42  MAP Kinase (ERK2) antibody shows detection of ERK2 in several whole cell lysates:  HeLa (lane 1), A431 (lane 2), and NIH3T3 (lane 3).  Detection occurs using a 1:1,000 dilution of the primary antibody followed by 1:4,000 dilution of HRP Goat-a-Rabbit with visualization via ECL.  Film exposure was approximately 1’.  Other detection systems will yield similar results.
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Synonyms: MAP kinase 1, MAP kinase 2, p42-MAPK

Erk2 Antibody Properties

Description
Anti-Erk2 (RABBIT) Antibody - 100-401-218
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:2,000 to 1:10,000
Western Blot : 1:1,000-1:,2000
ImmunoPrecipitation: 5.0 µg/mg sample
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
MAPK1
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Lot#  

Erk2 Antibody Description

Background
Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK’s) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively.  When activated, ERK’s form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes.  As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli.  Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.  In vertebrates the mitogen-induced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.
Immunogen
Anti-Erk2 antibody was prepared by repeated immunizations with an Erk2 containing fusion protein.  The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein.  The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This antiserum was tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 42 kDa in size corresponding to p42 MAP Kinase (ERK2) by western blotting in the appropriate cell lysate or extract.  p42 MAP Kinase (ERK2) is a ubiquitous protein kinase target for Ras and Raf.  The following cell lines have been assayed by immunoblot and were found to be positive for p42 MAP Kinase (ERK2) using this reagent:  U937, HeLa,  NIH-3T3, RAW 264.7, LNCaP and HEK whole cell lysates.
Purity/Specifity
This antiserum is directed against human p42 MAP Kinase (ERK2) protein and is useful in determining its presence by immunoblotting.  No reactivity is observed against p44 MAP Kinase (ERK1).  Cross reactivity is expected with p44 MAP Kinase (ERK1) proteins from human and mouse sources.  Reactivity to rat tissues is also anticipated due to high sequence homology.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Pelech, SL and Charest, DL (1995) MAP kinase-dependent pathways in cell cycle control. Prog Cell Cycle Res 1(11):33-52. Garrington, TP and Johnson, GL (1999) Organization and regulation of mitogen-activated protein kinase signaling pathways. Curr Opin Cell Biol 11(2) Apr, 211-218. Elion, EA (1998) Routing MAP kinase cascades. Science 281(5383):1625-1626.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-281

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
600-401-905

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-928

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry