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RFX5 Antibody

Rabbit Polyclonal
NCI Collaboration
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Number:
100-401-194
Size:
100 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

RFX5 Antibody Properties

Description
Anti-RFX5 (RABBIT) Antibody - 100-401-194
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:25,000
Western Blot : 1:500 - 1:3,000
Other Dilution: User Optimized
Species of Origin
human
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
RFX5
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RFX5 Antibody Description

Background
RFX5 Antibody detects the RFX5 protein. Regulatory factor X subunit 5 (RFX5) is a member of a family of DNA-binding proteins that share a novel and highly characteristic DNA-binding domain called the RFX motif. It mediates cooperative binding between RFX and NF-Y, recognizes X-boxes, and activates transcription from class II MHC promoters. RFX5 mutations are seen in cases of a severe immunodeficiency syndrome called MHC-II deficiency (also known as bare lymphocyte syndrome (BLS)). These mutations prevent the RFX complex from binding to the X box in MHC-II promoters, resulting in a lack of MHC-II expression. Anti-RFX5 antibody is ideal for investigators involved in cytokines and growth factor research.
Synonyms
DNA binding protein RFX5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, MHC
Immunogen
RFX5 peptide corresponding to amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-RFX5 Antibody was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 µl per assay. Specific conditions should be optimized by user. Anti-RFX5 Antibody was assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG. Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5. Other asssays should be optimized by researcher.
Purity/Specifity
Anti-RFX5 Antibody was prepared by repeated immunizations of an RFX5 peptide conjugate and purified as monospecific antiserum after delipidation and defibrination.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Reith W, Mach B (2001). "The bare lymphocyte syndrome and the regulation of MHC expression". Annu. Rev. Immunol. 19: 331–73.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-191

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-193

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-401-191

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-401-193

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry