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p27 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western blot using Rockland's affinity purified anti-p27 antibody shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead).  Separation was achieved using a 4-20% gradient gel.  Blocking occurred using 5% BLOTTO.  Primary antibody was diluted 1:500 in 1% BLOTTO.  The membrane was washed and reacted with a 1:10,000 dilution of Dylight™ 800 conjugated Gt-a-Rabbit IgG.  Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M).  Other detection systems will yield similar results.
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Number:
100-401-172
Size:
100 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

p27 Antibody Properties

Description
Anti-p27 (RABBIT) Antibody - 100-401-172
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:40,000
Western Blot : 1:1,000 - 1:4,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CDKN1B
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p27 Antibody Description

Background
p27 Kip is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21.  p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Synonyms
Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length recombinant human p27 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This antibody is suitable for use in ELISA, immunoprecipitation, co-immunoprecipitation and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kDa in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. No reactivity is observed with p27 from mouse. This antibody is suitable for immmunoprecipitation of in vitro translated protein and cell lysates (C212, 3T3-L1) and will co-precipitate associated proteins. Optimal titers for other applications should be determined by the researcher.
Purity/Specifity
This product was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with a p27 protein from human tissue.  No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse.  Reactivity against p27 from other species is unknown.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Keller,U.B., Old,J.B., Dorsey,F.C., Nilsson,J.A., Nilsson,L., MacLean,K.H., Chung,L., Yang,C., Spruck,C., Boyd,K., Reed,S.I. and Cleveland,J.L. (2007) Myc targets Cks1 to provoke the suppression of p27Kip1, proliferation and lymphomagenesis. EMBO J. 26 (10), 2562-2574. Naidu,R., Har,Y.C. and Taib,N.A. (2007) P27 V109G Polymorphism is associated with lymph node metastases but not with increased risk of breast cancer. J. Exp. Clin. Cancer Res. 26 (1), 133-140. Pertia,A., Nikoleishvili,D., Trsintsadze,O., Gogokhia,N., Managadze,L. and Chkhotua,A. Prognostic significance of p27(Kip 1) expression in renal cell Carcinoma. Georgian Med News 143, 12-17.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
Human
Catalog Number
100-401-156

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-162

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry