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Primary Antibodies > Cell Cycle Protein Antibodies

p27 Antibody

Rabbit Polyclonal

Code

100-401-172

Size

100 µL

Price

$294.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-p27 antibody shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight™ 800 conjugated Gt-a-Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.

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Synonyms: Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1

Datasheet
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p27 Antibody Properties

Description

Anti-p27 (RABBIT) Antibody - 100-401-172

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:10,000 - 1:40,000
Western Blot : 1:1,000 - 1:4,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

Antiserum

Label

Unconjugated

Concentration

80 mg/mL by Refractometry

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

CDKN1B

p27 Antibody Description

Background

p27 Kip is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length recombinant human p27 protein.

Immunogen Type

Recombinant Protein

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This antibody is suitable for use in ELISA, immunoprecipitation, co-immunoprecipitation and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kDa in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. No reactivity is observed with p27 from mouse. This antibody is suitable for immmunoprecipitation of in vitro translated protein and cell lysates (C212, 3T3-L1) and will co-precipitate associated proteins. Optimal titers for other applications should be determined by the researcher.

Purity/Specifity

This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse. Reactivity against p27 from other species is unknown.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Keller,U.B., Old,J.B., Dorsey,F.C., Nilsson,J.A., Nilsson,L., MacLean,K.H., Chung,L., Yang,C., Spruck,C., Boyd,K., Reed,S.I. and Cleveland,J.L. (2007) Myc targets Cks1 to provoke the suppression of p27Kip1, proliferation and lymphomagenesis. EMBO J. 26 (10), 2562-2574. Naidu,R., Har,Y.C. and Taib,N.A. (2007) P27 V109G Polymorphism is associated with lymph node metastases but not with increased risk of breast cancer. J. Exp. Clin. Cancer Res. 26 (1), 133-140. Pertia,A., Nikoleishvili,D., Trsintsadze,O., Gogokhia,N., Managadze,L. and Chkhotua,A. Prognostic significance of p27(Kip 1) expression in renal cell Carcinoma. Georgian Med News 143, 12-17.

Specific Reference

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra