House Secondary Banner
 
You may also want to consider these related products.
Anti-Cyclin A (RABBIT) Antibody - 100-401-151
Anti-Cyclin A (RABBIT) Antibody - 100-401-151
Anti-cdk4 (RABBIT) Antibody - 100-401-162
Anti-cdk4 (RABBIT) Antibody - 100-401-162
Anti-RABBIT (H&L) (GOAT) Antibody Fluorescein Conjugated - 211-1202
Anti-RABBIT (H&L) (GOAT) Antibody Fluorescein Conjugated - 211-1202
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Primary Antibodies  >  Cell Cycle Protein Antibodies
 

p16 Antibody

Rabbit Polyclonal
Code

100-401-170
Size

100 µL
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Rockland's Anti-p16 antibody was diluted 1:500 to detect p16 in human prostate tissue.  Tissue was formalin fixed and paraffin embedded.  No pre-treatment of sample was required.  The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3 Cyclin-dependent kinase 4 inhibitor A, CDK4I, p16-INK4a, Short name=p16INK4A, p16-INK4, Multiple tumor suppressor 1=MTS-1

p16 Antibody Properties

Description
Anti-p16 (RABBIT) Antibody - 100-401-170
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:5,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CDKN2A, CDKN2, MTS1
Download CofA
Lot#  

p16 Antibody Description

Background
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene (isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Immunogen
human p16 protein (missing the first 7 amino acids)
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This product was assayed by immunoblot and found to be reactive against p16 from cell lysates and E.coli produced fusion proteins at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (code #611-1302).  Anti-p16 is suitable for the detection by immunoblot of human p16.  This antisera is also suitable for immmunoprecipitation of in vitro translated protein and cell lysates (Saos-2, 3T3-L1 weakly) and will co-precipitate associated proteins including p15 and potentially p15.5. This product was assayed by immunoblot and found to be reactive against p16 from cell lysates and E.coli produced fusion proteins at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (code #611-1302).  Anti-p16 is suitable for the detection by immunoblot of human p16.  This antisera is also suitable for immmunoprecipitation of in vitro translated protein and cell lysates (Saos-2, 3T3-L1 weakly) and will co-precipitate associated proteins including p15 and potentially p15.5. 
Purity/Specifity
This product was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with a p16 protein from human  tissue.  Cross reactivity may occur with p15 when immunoprecipitating in vitro translated product but not in immunoblots.  Potentially cross reacts with p15.5.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Serrano et al., (1993) Nature 366; 704-707.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, mouse, rat
Catalog Number
100-401-151

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-162

Product Type
Secondary Antibodies;
Applications
FLISA, IF, FC
Catalog Number
211-1202

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry