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Cdk9 Antibody

Rabbit Polyclonal
NCI Collaboration
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Immunocytochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum.  The staining shows the location of mcdk9/PITALRE protein in developing mouse tissue. Arrows indicate areas of high expression.  Panel A: Peroxidase-DAB immunostaining of mcdk9/PITALRE protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle.  Similar staining is shown in Panel B in the dorsal root ganglia.   Panel C:  Fluorescein immunofluorescence of mcdk9IPITALRE in skeletal muscle.   Similar staining is shown in Panel D in cardiac muscle.   Other detection systems should yield similar results.  Sections from each specimen were cut at 5-7 µm, mounted on glass and dried overnight at 37°C. All sections then were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. Slides were incubated at 20° C for 1 h  with  rabbit anti-cdk9 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. All the slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C.  Diaminobenzidine was used as the final chromogen and hematoxylin was used as the nuclear counterstain. Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.
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Number:
100-401-167
Size:
100 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Cdk9 Antibody Properties

Description
Anti-cdk9 (PITALRE) (RABBIT) Antibody - 100-401-167
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, rat and mouse
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:50,000
Western Blot : 1:500 - 1:3,000
Immunohistochemistry: 1:200 - 1:1,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CDK9
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Cdk9 Antibody Description

Background
CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2.   This kinase also was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation.  This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation.  Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb.   The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev.  CDK9 (PITALRE) is a ubiquitously expressed nuclear protein.
Synonyms
Cell division cycle 2 like protein kinase 4 antibody, Cell division protein kinase 9 antibody, Cyclin dependent kinase 9 antibody, PITALRE antibody, Serine threonine protein kinase PITALRE antibody, TAK antibody
Immunogen
Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa corresponding to CDK9 (PITALRE) by western blotting in the appropriate cell lysate or extract. HeLa cells may be used as a positive control.
Purity/Specifity
This product was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with a 43 kDa cdk9 (PITALRE) protein from human, rat and mouse tissue.  No reaction was observed against other related cyclin dependent kinases.  Cross reactivity with cdk9 (PITALRE) from other species may also occur.  The murine cDNA is shown to be 98% identical with human. For immunohistochemistry use paraffin embedded tissue.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Liu,H. and Herrmann,C.H. (2005) Differential localization and expression of the Cdk9 42k and 55k isoforms. J. Cell. Physiol. 203 (1), 251-260. Peng, J., Zhu, Y., Milton, J.T. and Price, D.H. (1998). Genes Dev. 12(5):755-762. Zhou, Q., Chen, D., Pierstorff, E and Luo, K. (1998) Transcription elongation factor P-TEFb mediates Tat activation of HIV-1 transcription at multiple stages. EMBO J. 17(13):3681-3691.
Specific Reference
Graña, X., DeLuca, A., Sang, N., Fu, Y., Claudio, P., Rosenblatt, J., Morgan, D. and Giordano, A. (1994) PITALRE a nuclear cdc2-related protein kinase that phosphorylates the retinoblastoma protein in vitro. Proc. Natl. Acd Sci. USA 91; 3834-3838. Bullrich, F., MacLachlan, T., Sang, N., Druck, T., Veronese, M.L., Allen, S., Chirazzi, N., Koff, A., Heubner, K., Croce, C. and Giordano, A. (1995) Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, PITALRE and a cdk inhibitor, p27, to regions involved in human cancer. Cancer Res. 55; 1199-1205. Bagella, L., MacLachlan, T.K., Buono, R.J., Pisano, M.M., Giordano, A. and De Luca, A. (1998) Cloning of murine cdk9/PITALRE and its tissue-specific expression in development. J. Cell Physiol. 177(2):206-13. MacLachlan, T.K., Sang, N., De Luca, A.., Puri, P.L., Levrero, M. and Giordano, A. and (1998) Binding of cdk9 to TRAF2. J. Cell Biochem. 71(4):467-478. De Falco, G. and Giordano, A. (1998) CDK( (PITALRE): A Multifunctional cdc-2-related Kinase. J. Cell. Physiol. 177; 501-506. Fujinaga, K., Cujec, T.P., Peng, J., Garriga, J., Price, D.H., Graña, X. and Peterlin, B.M. (1998) The ability of positive transcription elongation factor B to transactivate human immunodeficiency virus transcription depends on a functional kinase domain, cyclin T1 and Tat. J. Virol. 72(9) 7154-7159.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-161

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-162

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Human
Catalog Number
200-401-410

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-897

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry