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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

Cdk5 Antibody

Rabbit Polyclonal
Code

100-401-163
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Rockland's anti-CDK5 antibody was diluted 1:500 to detect CDK5 in human brain cortex tissue.  Tissue was formalin fixed and paraffin embedded.  No pre-treatment of sample was required.  The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Synonyms: Cell division protein kinase 5 antibody, Crk6 antibody, Cyclin dependent kinase 5 antibody, Protein kinase CDK5 splicing antibody, PSSALRE antibody, Serine threonine protein kinase PSSALRE antibody

Cdk5 Antibody Properties

Description
Anti-cdk5 (RABBIT) Antibody - 100-401-163
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, rat and mouse
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:5,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
85 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CDK5
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Lot#  

Cdk5 Antibody Description

Background
The protein encoded by the gene CDK5 is involved in the control of the cell cycle and interacts with D1 and D3-type G1 cyclins. CDK5 belongs to the protein kinase superfamily, the CMGC Ser/Thr protein kinase family and the CDC2/CDKX subfamily. CDK5 phosphorylates histone H1, tau, MAP2 and NF-H and NF-M and also interacts with p35 which activates the kinase. CDK5 forms a heterodimer, where CDK5 is the catalytic subunit and p35 is the regulatory subunit. CDK5 is also found in a trimolecular complex with CABLES1 and ABL1. CDK5 is a cytoplasmic protein.
Immunogen
cdk5 (p31) peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This product was assayed by immunoblot and found to be reactive against cdk5 (p31) at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  Anti-cdk5 (p31) is suitable for the detection by immunoblot of human, rat and mouse cdk5 (p31).  Suggested dilutions for ELISA are 1:500 to 1:2000. 
Purity/Specifity
This product was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with a 35 kDa cdk5 (p31) protein from
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Meyerson, M., Enders, G.H., Su, L.K., Gorka, C., Nelson, C., Harlow, E. and Tsai, L.H. (1992) A family of cdc2-related protein kinases. EMBO J. 11; 2909-2917. Hellmich, M.R., Pant, H.C., Wada, E. and Battey, J.F. (1992) Neuronal cdc2-like kinase: a cdc2-related protein kinase with predominantly neuronal expression. PNAS USA 89; 10867-10871.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human
Catalog Number
100-401-153

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-160

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-161

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-167

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry