House Phospho Banner
 
You may also want to consider these related products.
Anti-cdk9 (PITALRE) (RABBIT) Antibody - 100-401-167
Anti-cdk9 (PITALRE) (RABBIT) Antibody - 100-401-167
Anti-Erk2 (RABBIT) Antibody - 100-401-218
Anti-Erk2 (RABBIT) Antibody - 100-401-218
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 649 Conjugated - 611-143-002
Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 649 Conjugated - 611-143-002
Primary Antibodies  >  Cell Cycle Protein Antibodies
 

Cyclin T1 Antibody

Rabbit Polyclonal
Code

100-401-158
Size

100 µL
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Amino acids are numbered on the bottom of each protein. The cyclin box is indicated. Human cyclins T2a and T2b have 642 amino acids in common but different carboxyl termini (black boxes).       Background Information:  Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation.  This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb.   P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev.  Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.
Diagram of human cyclins T1, T2a, and T2b and Drosophila cyclin T. Amino acids are numbered on the bottom of each protein. The cyclin box is indicated. Human cyclins T2a and T2b have 642 amino acids in common but different carboxyl termini (black boxes).
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: cyclin-T1 antibody, CYCT 1 antibody, cycT1 antibody, HIVE1 antibody, Human immunodeficiency virus 1 expression antibody

Cyclin T1 Antibody Properties

Description
Anti-Cyclin T1 (RABBIT) Antibody - 100-401-158
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 1:200 - 1:1,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
75 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CCNT1
Download CofA
Lot#  

Cyclin T1 Antibody Description

Background
Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation.  This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb.   P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev.  Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.
Immunogen
Cyclin T1 peptide corresponding to an internal region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Suitable for ELISA, immunoprecipitation, immunohistochemistry, immunoblotting and other immunological methods requiring high titer and specificity.  Anti-Cyclin T1 is suitable for the detection by immunoblot of human, rat and mouse Cyclin T1.  Use paraffin embedded tissue for immunohistochemistry. HeLa cells may be used as a positive control.
Purity/Specifity
This product was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with an 85 kDa cyclin T1 protein from human, rat and mouse tissue.  No reaction was observed against other related cyclins.  Cross reactivity with cyclin T1 from other species may also occur.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Bagella, L., MacLachlan, T.K., Buono, R.J., Pisano, M.M., Giordano, A. and De Luca, A. (1998) Cloning of murine cdk9/PITALRE and its tissue-specific expression in development. J. Cell Physiol. 177(2):206-13. MacLachlan, T.K., Sang, N., De Luca, A.., Puri, P.L., Levrero, M. and Giordano, A. and (1998) Binding of cdk9 to TRAF2. J. Cell Biochem. 71(4):467-478. Peng, J., Zhu, Y., Milton, J.T. and Price, D.H. (1998). Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 12(5):755-762. De Falco, G. and Giordano, A. (1998) CDK( (PITALRE): A Multifunctional cdc-2-related Kinase. J. Cell. Physiol. 177; 501-506. Fujinaga, K., Cujec, T.P., Peng, J., Garriga, J., Price, D.H., Graña, X. and Peterlin, B.M. (1998) The ability of positive transcription elongation factor B to transactivate human immunodeficiency virus transcription depends on a functional kinase domain, cyclin T1 and Tat. J. Virol. 72(9) 7154-7159.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-167

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-218

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Secondary Antibodies;
Applications
FLISA, WB, IF
Reacts With
Rabbit
Catalog Number
611-143-002

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry