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Primary Antibodies > Cell Cycle Protein Antibodies

Cyclin E Antibody

Rabbit Polyclonal

Code

100-401-156

Size

100 µL

Price

$294.00

Availability

Ships next business day.

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Shipping:

$85.00 to United States

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Synonyms: CCNE antibody, CCNE1 antibody, Cyclin E1 antibody, Cyclin Es antibody, Cyclin Et antibody, CyclinE antibody, G1/S specific cyclin E antibody

Datasheet
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Cyclin E Antibody Properties

Description

Anti-Cyclin E (RABBIT) Antibody - 100-401-156

Host

Rabbit

Target Species

Human

Known Cross Reactivity

Human

Clonality

Polyclonal

Application

ELISA : 1:100,000
Western Blot : 1:2,000 - 1:10,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

Antiserum

Label

Unconjugated

Concentration

70 mg/mL by Refractometry

Buffer

None

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

CCNE1

Cyclin E Antibody Description

Background

Anti-Cyclin-E antibody detects cyclin-E. Cyclin E is a member of the cyclin family. Cyclin E binds to the G1 phase Cdk2, which is required for the transition from G1 to S phase of the cell cycle that determines cell division. The Cyclin E/CDK2 complex phosphorylates p27Kip1, tagging it for degradation, thus promoting expression of Cyclin A, allowing progression to S phase. Anti-cyclin-e Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.

Immunogen

Anti-Cyclin-E Antibody was produced by repeated immunizations with a KLH conjugated peptide corresponding to a region near the carboxy terminus of human Cyclin E.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

Anti-Cyclin-E antibody is suitable for the detection by immunoblot, ELISA and IP. Researchers should determine optimal titers for applications that are not stated below. Antiserum will specifically react with a 45 kDa Cyclin E protein from human tissue.

Purity/Specifity

Anti-Cyclin-E was prepared from monospecific antiserum by delipidation and defibrination. No reaction was observed against other related cyclins. Cross reactivity with Cyclin E from other species may also occur.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Specific Reference

Koff, A., Cross, F., Fisher, A., Schumaker, J., Leguellec, K., Philippe, M. and Roberts, J.M. (1991) Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. Cell 66; 1217-1228. Koff, A., Giordano, A., Desai, D., Yamashita, K., Harper, J.W., Elledge, S., Nishimoto, T., Morgan, D.O., Franza, B.R. and Roberts, M. (1992) Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle. Science 257; 1689-1694. Claudio, P.P., De Luca, A., Howard, C.M., Baldi, A., Firpo, E.J., Koff, A., Paggi, M.G. and Giordano, A. (1996) Functional analysis of pRb/p130 intereaction with cyclins. Cancer Res. 56; 2003-2008.

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AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

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DyLight™ Antibody Spectra

ELISA