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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

Cyclin B1 Antibody

Rabbit Polyclonal
Code

100-401-152
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western blot analysis using Rockland's Anti-Cyclin B<sub>1</sub> antibody shows detection of human Cyclin B<sub>1</sub> present in asynchronous HN30 cell lysates.  HN30 cells are from head and neck cancer tumors that over express cyclin B<sub>1</sub> and D<sub>1</sub>. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein (arrowhead).  The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature.  Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG 611-103-122 and chemiluminescence reagent with a 1-min exposure time.  Other detection systems will yield similar results. Personal communication, Luca Cote, Temple University, Philadelphia, PA.
Western blot analysis using Rockland's Anti-Cyclin B<sub>1</sub> antibody shows detection of Cyclin B<sub>1</sub> present in asynchronous HeLa cell lysates.  Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B<sub>1</sub> (arrowhead).  Approximately 50 µg of lysate was loaded on to a 7% SDS-PAGE gel for separation. After transfer to nitrocellulose, the blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature.  Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG (p/n 611-103-122).  Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.
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Synonyms: G2/mitotic-specific cyclin-B1

Cyclin B1 Antibody Properties

Description
Anti-Cyclin B1 (RABBIT) Antibody - 100-401-152
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, rat, mouse
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500 - 1:1,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CCNB1
Download CofA
Lot#  

Cyclin B1 Antibody Description

Background
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Immunogen
Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This antibody is suitable for use in ELISA, immunoblotting, immunoprecipitation and other immuno-logical methods requiring high titer and specificity.   Specific conditions for reactivity and detection of Cyclin B1 should be optimized by the end user.  Expect a band approximately 55-66 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. H23 cells may be used as a positive control.
Purity/Specifity
This product was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue.  No reaction was observed against other related cyclins.  Cross reactivity with Cyclin B1 from other species may also occur.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Giordano, A., Whyte, P., Harlow, E., Franza, B., Beach D. and Draetta, G. (1989) A 60 kd cdc-2 associated polypeptide complexes with the E1A proteins of adenovirus-infected cells. Cell 58; 981-990. Pines, J. and Hunter, T. (1990) Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. Nature 346; 760-763.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human
Catalog Number
100-401-153

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Human, Mouse
Catalog Number
600-401-398

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry