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Primary Antibodies  >  Serum Protein Component Antibodies
 

Alpha-2-Macroglobulin Antibody

Goat Polyclonal
Code

100-101-207
Size

2 mL
Price

$309.00
Availability

Ships next business day.
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Rockland Goat anti Alpha-2-Macroglobulin (200-101-207 lot 20172) was used to detect Alpha-2-Macroglobulin    under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes.  Samples of ~1ug of protein per lane were run by SDS-PAGE.  Protein was transferred to nitrocellulose and probed with  1:3000 dilution of primary antibody (ON 4 C in MB-070).  Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
Western Blot of Goat anti-Alpha-2-Macroglobulin Antibody.  
Lane 1:  Alpha-2-Macroglobulin.  
Lane 2:  none.  
Load:  100 ng per lane.
Primary antibody:  Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4°C.
Secondary antibody:  DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size:  163 kDa, 178 kDa for Alpha-2-Macroglobulin. 
Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody

Alpha-2-Macroglobulin Antibody Properties

Description
Anti-alpha-2-MACROGLOBULIN (Human Plasma) (GOAT) Antibody - 100-101-207
Host
Goat
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
82 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
A2M
Download CofA
Lot#  

Alpha-2-Macroglobulin Antibody Description

Immunogen
a2-Macroglobulin [Human Plasma]
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This product has been assayed against 1.0 ug of a2-Macroglobulin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:32,000 to 1:160,000 of the reconstitution concentration is suggested for this product.
Purity/Specifity
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified a2-Macroglobulin [Human Plasma].  Cross reactivity against a2-Macroglobulin from other sources is unknown.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-101-208

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-101-240

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, monkey
Catalog Number
600-401-B22

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
605-9502

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry