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Primary Antibodies  >  Serum Protein Component Antibodies
 

Alpha 1 Anti Trypsin Antibody

Goat Polyclonal
Code

100-101-147
Size

2 mL
Price

$309.00
Availability

Ships next business day.
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Rockland primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (1° 109-4134, green 2° 611-743-127); Alpha 1 anti trypsin (1° 100-101-147, red 2° 605-742-125); and Human IgG (1° 109-3102,  Blue 2° 610-741-124 in a multiplex fluorescent western blot of human serum.
Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT.  Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~ 1hr at 4 degrees.   After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Biorad VersaDoc4000.
Rockland Goat anti Alpha-1anti-Trypsin antibody (200-101-147 lot 5854) was used to detect Alpha-1anti-Trypsin under reducing (R) conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes.  Samples of ~1ug of protein per lane were run by SDS-PAGE.  Protein was transferred to nitrocellulose and probed with  1:3000 dilution of primary antibody (ON 4 C in MB-070).  Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibod

Alpha 1 Anti Trypsin Antibody Properties

Description
Anti-ALPHA-1-ANTI-TRYPSIN (Human Plasma) (GOAT) Antibody - 100-101-147
Host
Goat
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
Antiserum
Label
Concentration
90 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SERPINA1
Download CofA
Lot#  

Alpha 1 Anti Trypsin Antibody Description

Immunogen
a1-Anti-Trypsin [Human Plasma]
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This product has been assayed against 1.0 ug of a1-Anti-Trypsin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:7,000 to 1:30,000 of the reconstitution concentration is suggested for this product.
Purity/Specifity
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified a1-Anti-Trypsin [Human Plasma].  Cross reactivity against a1-Anti-Trypsin from other sources is unknown.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
200-103-147

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-147

Product Type
Secondary Antibodies;
Catalog Number
605-9302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
605-9602

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry