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Proteins and Peptides  >  Purified Proteins
 

Monkey IgM

Code

017-0107
Size

250 µg
Price

$199.00
Availability

Ships next business day.
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SDS-PAGE of Monkey IgM Whole Molecule. Lane 1: Monkey IgM, Non-Reduced. Lane 2: Monkey IgM, Reduced. Load: 1.0 µg per lane. Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.
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Synonyms: Monkey Immunoglobulin M

Monkey IgM Properties

Description
MONKEY IgM whole molecule - 017-0107
Application
ELISA : User Optimized
Western Blot : User Optimized
Immunochemistry: User Optimized
Other Dilution: User Optimized
Species of Origin
Monkey
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgM
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.09 mg/mL by UV absorbance at 280 nm
Buffer
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

Monkey IgM Description

Background
Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Storage Condition
Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Application Note
Monkey IgM whole molecule can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments. Monkey IgM whole molecule is stable at 4° C prior to restoration. It is recommended to aliquot restored Monkey IgM whole molecule after adding equal parts glycerol for storage at -20° C for extended storage and to prevent repeated freeze-thaw cycles.
Purity/Specifity
Monkey IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  Monkey IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Monkey Serum and anti-Monkey IgM (µ chain specific).   No reaction was observed against anti-Monkey IgG F(c).  Some light chain cross reactivity will occur with anti-Monkey IgG.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
009-0007

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC, IP, FC
Catalog Number
010-0102

Product Type
Secondary Antibodies;
Applications
FLISA, IF, FC
Catalog Number
617-100-007

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry