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Mouse IgG2b isotype Control

Monoclonal M2B IgG2b
NCI Collaboration
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Number:
010-0142
Size:
1 mg
Price:
$169.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Mouse IgG2b isotype Control Properties

Description
MOUSE IgG2b isotype control - 010-0142
Clonality
Monoclonal M2B IgG2b
Application
ELISA : User Optimized
FLISA : User Optimized
IF Microscopy : User Optimized
Flow Cytometry : 1:1000-1:5000
Western Blot : User Optimized
Other Dilution: User Optimized
Species of Origin
Mouse
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG2b
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.1 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Mouse IgG2b isotype Control Description

Background
Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Synonyms
MOUSE IgG2b subclass isotype control, MOUSE IgG2b isotype control
Storage Condition
Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Mouse IgG2b isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Purity/Specifity
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.   Extensive cross-adsorbtion was performed to remove any unwanted subclasses.  Typically less than 1% cross reactivity against other mouse and human heavy or light chains isotypes was detected by ELISA.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC, IP, FC
Catalog Number
010-0102

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry