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MOUSE IgM

NCI Collaboration
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SDS-PAGE of Mouse IgM Whole Molecule. Lane 1: Mouse IgM, Non-Reduced. Lane 2: Mouse IgM, Reduced. Load: 1.0 µg per lane. Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.
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Number:
010-0107
Size:
1 mg
Price:
$169.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

MOUSE IgM Properties

Description
MOUSE IgM whole molecule - 010-0107
Application
ELISA : User Optimized
Western Blot : User Optimized
Other Dilution: User Optimized
Species of Origin
Mouse
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgM
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.127 by UV absorbance at 280 nm
Buffer
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
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MOUSE IgM Description

Background
Mouse IgM, or mouse Immunoglobulin M, purified protein is a basic antibody that is produced by B cells. Mouse IgM is the primary antibody against A and B antigens on red blood cells. Mouse IgM is by far the physically largest antibody in the human circulatory system. Mouse IgM is the first antibody to appear in response to initial exposure to antigen.
Synonyms
Mouse Immunoglobulin M
Storage Condition
Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Application Note
Mouse IgM whole molecule can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Purity/Specifity
Mouse IgM was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (µ chain specific).   No reaction was observed against anti-Mouse IgG F(c).  Some light chain cross-reactivity will occur with anti-Mouse IgG.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC, IP, FC
Catalog Number
010-0102

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry