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Proteins and Peptides  >  Purified Proteins
 

Mouse IgG Fab fragment

Code

010-0105
Size

2 mg
Price

$159.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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SDS-PAGE of Mouse IgG Fab Fragment (p/n 010-0105). Lane 1: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 2: Reduced Mouse IgG Whole Molecule (p/n 010-0102). Lane 3: Reduced Mouse F(c) Fragment (p/n 010-0103). Lane 4: Reduced Mouse F(ab) Fragment (p/n 010-0105). Lane 5: Mouse IgM Kappa Myeloma Protein (p/n 010-0107). Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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Synonyms: Mouse Immunoglobulin Fab, F(ab), Fragment antigen-binding

Mouse IgG Fab fragment Properties

Description
MOUSE IgG Fab fragment - 010-0105
Application
ELISA : User Optimized
Western Blot : User Optimized
Immunochemistry: User Optimized
Other Dilution: User Optimized
Species of Origin
Mouse
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG Fab
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

Mouse IgG Fab fragment Description

Background
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain. Mouse IgG Fab Fragment is ideal for investigators in Immunology, Cancer, and Microbiology research.
Storage Condition
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Application Note
Mouse IgG Fab Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Purity/Specifity
Mouse IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.  Mouse IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG, anti-Mouse IgG F(ab’)2 and anti-Mouse Serum.  No reaction was observed against anti-Mouse IgG F(c) or anti- Papain.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Janeway, Jr., Travers, Walport, and Shlomchik. "The Immune System in Health and Disease." Immunobiology, 5th Edition: Garland Science: 2001.
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
006-0105

Product Type
Proteins and Peptides;
Catalog Number
010-0205

Product Type
Proteins and Peptides;
Catalog Number
010-0305

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
011-0105

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry