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Proteins and Peptides  >  Purified Proteins
 

MOUSE IgG F(c)

Code

010-0103
Size

1 mg
Price

$195.00
Availability

Ships next business day.
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SDS-PAGE of Mouse IgG F(c) Fragment (p/n 010-0103). Lane 1: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 2: Reduced Mouse IgG Whole Molecule (p/n 010-0102). Lane 3: Reduced Mouse F(c) Fragment (p/n 010-0103). Lane 4: Reduced Mouse F(ab) Fragment (p/n 010-0105). Lane 5: Mouse IgM Kappa Myeloma Protein (p/n 010-0107). Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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Synonyms: MOUSE IgG F(c) fragment, Mouse Fc, Immunoglobulin Fc

MOUSE IgG F(c) Properties

Description
MOUSE IgG F(c) fragment - 010-0103
Application
ELISA : User Optimized
Western Blot : User Optimized
Immunochemistry: User Optimized
Other Dilution: User Optimized
Species of Origin
Mouse
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG F(c)
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
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MOUSE IgG F(c) Description

Background
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The mouse F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the mouse F(c) fragment no longer posses the epitope recognition site.
Storage Condition
Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Mouse IgG Fc fragment is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Mouse IgG F(c) Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Mouse IgG F(c) Fragment is stable at 4° C prior to restoration. It is recommended to aliquot restored Mouse IgG F(c) Fragment and store at -20° C for extended storage and to prevent repeated freeze-thaw cycles.
Purity/Specifity
MOUSE IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(c).  No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
001-0103

Product Type
Proteins and Peptides;
Catalog Number
004-0303

Product Type
Proteins and Peptides;
Catalog Number
010-0051

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
110-1103

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry