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MOUSE IgG1 Isotype control

Monoclonal MG1K IgG1
NCI Collaboration
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Number:
010-001-330
Size:
1 mg
Price:
$155.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

MOUSE IgG1 Isotype control Properties

Description
MOUSE IgG1 Kappa (κ) isotype control - 010-001-330
Clonality
Monoclonal MG1K IgG1
Application
ELISA : User Optimized
FLISA : User Optimized
IF Microscopy : User Optimized
Flow Cytometry : 1:1000-1:5000
Western Blot : User Optimized
Other Dilution: User Optimized
Species of Origin
Mouse
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG1
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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MOUSE IgG1 Isotype control Description

Background
Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain a - IgA, ? - IgG 1, 2a, 2b, 3 and µ - IgM, light chain ? and ?.
Synonyms
Mouse Isotype Control
Storage Condition
Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Application Note
Mouse IgG1 kappa isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Purity/Specifity
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.  In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3 , IgM , and IgA.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum.  Light and heavy chain composition has been confirmed..
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC, IP, FC
Catalog Number
010-0102

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry