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Proteins and Peptides  >  Purified Proteins
 

Human IgM (myeloma) (BULK ORDER)

Code

009-0107
Size

2 mg
Price

Price On Request
Availability

Ships next business day.
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Synonyms: Human immunoglobulin M

Human IgM (myeloma) (BULK ORDER) Properties

Description
HUMAN IgM (myeloma) whole molecule (BULK ORDER) - 009-0107
Application
ELISA : User Optimized
Western Blot : User Optimized
Immunochemistry: User Optimized
Other Dilution: User Optimized
Species of Origin
Human
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgM
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
10.2 mg/mL by UV absorbance at 280 nm
Buffer
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preservative
0.1% (w/v) Sodium Azide
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Human IgM (myeloma) (BULK ORDER) Description

Background
Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Storage Condition
Store vial at 4° C prior to opening.   Human IgM (myeloma) whole molecule is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Application Note
Human IgM (myeloma) whole molecule can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting.
Purity/Specifity
Human IgM (myeloma) whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  Human IgM (myeloma) whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgM (µ chain specific).  No reaction was observed against anti-Human IgG F(c).  Some light chain cross-reactivity will occur with anti-Human IgG.
Disclaimer Note-Human
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, WB
Catalog Number
010-0107

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
017-0107

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
109-4107

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
209-4307

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry