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Proteins and Peptides  >  Purified Proteins
 

Human IgG Fab

Code

009-0105
Size

2 mg
Price

$159.00
Availability

Ships next business day.
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SDS-Page of Human Fab.
Lane 1:  Human Fab - Non-Reduced.
Lane 2:  Human Fab - Reduced.
Load:  1.0 ug per lane.
Predicted/Observed size - Non-Reduced:  50 kDa, 50 kDa for Human Fab. 
Predicted/Observed size - Reduced:  25 kDa, 25 kDa for Human Fab. 
Other band(s): None.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Human Immunoglobulin Fab, F(ab), Fragment antigen-binding

Human IgG Fab Properties

Description
HUMAN IgG Fab fragment - 009-0105
Application
ELISA : User Optimized
Western Blot : User Optimized
Immunochemistry: User Optimized
Other Dilution: User Optimized
Species of Origin
Human
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG Fab
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
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Lot#  

Human IgG Fab Description

Background
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Human IgG Fab fragment is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Human IgG Fab Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Purity/Specifity
Human IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.  Human IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti- Human IgG and anti- Human IgG F(ab’)2.  No reaction was observed against anti- Human IgG F(c) or anti-Papain.
Disclaimer Note-Human
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
001-0105

Product Type
Proteins and Peptides;
Catalog Number
005-0305

Product Type
Proteins and Peptides;
Catalog Number
010-0005

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
209-1104

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry