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Proteins and Peptides  >  Collagen Proteins
 

COLLAGEN IV

Code

009-001-106
Size

500 µg
Price

$275.00
Availability

Ships next business day.
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Shipping:
$20.00 to United States
Handling:
$20.00
Synonyms:

COLLAGEN IV Properties

Description
Human COLLAGEN Type IV - 009-001-106
Application
ELISA : 1:10,000
Western Blot : 1:1000
Immunohistochemistry: 1:100-1:500
Other Dilution: User Optimized
Species of Origin
Human
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.0 mg/mL by dry weight
Buffer
0.5 M Acetic Acid
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
COL4A4
Download CofA
Lot#  

COLLAGEN IV Description

Background
Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6.
Storage Condition
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
For use as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.
Purity/Specifity
This product has been prepared from human placenta and is chromatographically and immunologically pure.  This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.  This product reacts with anti-Collagen Type IV.  Reaction with Rockland’s anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA).
Disclaimer Note-Human
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry