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Proteins and Peptides  >  Collagen Proteins
 

Human Collagen Type I

Code

009-001-103
Size

500 µg
Price

$245.00
Availability

Ships next business day.
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Western blot of Human Collagen Type I.
Lane 1:  Human Collagen Type 1.
Lane 2:  None.
Load:  50 ng per lane.
Primary antibody:  Collagen Type I antibody at 1:1,000 overnight at 4°C.
Secondary antibody:  DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  139 & 130 kDa, 139 & 130 kDa for Collagen Type I.
Other Band(s):  Collagen Type I splice variants and isoforms.
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Shipping:
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Handling:
$20.00
Synonyms: Type I collagen, collagen 1, human collagen

Human Collagen Type I Properties

Description
Human COLLAGEN Type I - 009-001-103
Known Cross Reactivity
human
Application
ELISA : 1:10,000
Western Blot : 1:1000
Immunochemistry: 1:100-1:500
Other Dilution: User Optimized
Species of Origin
Human
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.0 mg/mL by dry weight
Buffer
0.5 M Sodium Acetate, pH 4.5
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
COL1A1
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Lot#  

Human Collagen Type I Description

Background
Collagen type I is the fibrillar collagen found in most connective tissues, including cartilage. Collagen type I is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendon, skin and the white part of the eye (sclera).
Storage Condition
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Human Collagen type I purified protein standard is used as a control in ELISA and western blot. Collagen type I is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.
Purity/Specifity
Human Collagen type I has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.  Human Collagen type I reacts with anti-Collagen Type I.  Reaction of Human Collagen type I with Rockland’s anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA)
Disclaimer Note-Human
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
009-001-104

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
009-001-105

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
009-001-106

Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC
Catalog Number
009-001-107

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry