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Proteins and Peptides  >  Purified Proteins
 

Chicken IgG (BULK ORDER)

Code

003-0102
Size

10 mg
Price

Price On Request
Availability

Ships next business day.
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SDS PAGE of Chicken IgG Whole Molecule. Lane 1: Non-Reduced Chicken IgG Whole Molecule. Lane 2: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 3: Reduced Chicken IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-Reduced at 160kDa/Observed at 245 kDa; Reduced at 72, 25 kDa. Non-reduced IgG migrates slightly higher.
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Synonyms: Chicken immunoglobulin G

Chicken IgG (BULK ORDER) Properties

Description
CHICKEN IgG whole molecule (BULK ORDER) - 003-0102
Application
ELISA : User Optimized
Western Blot : User Optimized
Immunochemistry: User Optimized
Other Dilution: User Optimized
Species of Origin
Chicken
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
10.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Chicken IgG (BULK ORDER) Description

Background
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-afinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Storage Condition
Store vial at 4° C prior to restoration.   Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.   Expiration date is one (1) year from date of restoration.
Application Note
Chicken IgG whole molecule can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Purity/Specifity
Chicken IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed extensive dialysis against the buffer stated above.  Chicken IgG whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG and anti-Chicken Serum.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, WB, IHC, IP, FC
Catalog Number
010-0102

Product Type
Proteins and Peptides;
Applications
ELISA, WB
Catalog Number
012-0102

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry