SDS PAGE Results of Bovine IgG Whole Molecule

Datasheet

Bovine IgG

Name: Bovine IgG
Brand: Rockland Immunochemicals
Availability: OutOfStock

1 References

Item No. 001-0102

Size: 50 mg

Applications: SDS-PAGE, Other

Origin: Bovine

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50 mg $0.00

1000 mg $0.00

10 mg $135.00

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Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

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Specifications for Bovine IgG

Product Details

Description: Bovine IgG Whole Molecule (BULK ORDER) - 001-0102

Synonyms: Bovine IgG whole molecule, Bovine Immunoglobulin G, cow IgG

Species of Origin: Bovine

Format: IgG

Type: Native Protein

Target Details

Purity/Specificity: Bovine IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Bovine IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum and anti-Bovine IgG.

Application Details

Tested Applications: SDS-PAGE

Suggested Applications: Other - View References

Application Note: Bovine IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation

Physical State: Lyophilized

Concentration: 10.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Reconstitution Volume: 5.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Bovine IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (1)

Ong LC et al. (2014). Bacterial imaging with photostable upconversion fluorescent nanoparticles. Biomaterials.

Applications

Control

PubMed

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.