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Proteins and Peptides  >  Collagen Proteins
 

Bovine Collagen Type V

Code

001-001-107
Size

500 µg
Price

$215.00
Availability

Ships next business day.
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Handling:
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Synonyms: Extracellular Matrix Protein

Bovine Collagen Type V Properties

Description
Bovine COLLAGEN Type V - 001-001-107
Application
ELISA : 1:10,000
Western Blot : 1:1000
Immunochemistry: 1:100-1:500
Other Dilution: User Optimized
Species of Origin
Bovine
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
PSC Type
Protein
PSC Format
Native Protein
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.5 M Sodium Acetate, pH 4.5
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
COL5A1
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Lot#  

Bovine Collagen Type V Description

Background
In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known. Bovine Collagen Type V is ideal for investigators involved in extracellular matrix proteins.
Storage Condition
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
For use as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.
Purity/Specifity
Bovine COLLAGEN Type V has been prepared from Bovine Placenta and is chromatographically and immunologically pure.  This product reacts with anti-Collagen Type V.  Reaction with Rockland’s anti-Collagen I, II, III, IV or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Perumal, S.; Antipova, O. & Orgel, J. P. (2008). "Collagen fibril architecture, domain organization, and triple-helical conformation govern its proteolysis". PNAS 105 (8): 2824–2829.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry