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GFP and Epitopes  >  Antibodies to GST
 

Glutathione-S-Transferase (GST) Control Protein

Code

000-001-200
Size

100 µg
Price

$115.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
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SDS-PAGE of Glutathione-S-Transferase (GST) Control Protein
Lane 1:  Glutathione-S-Transferase.
Lane 2:  Opal Prestained Protein Standard 10-245kDa.  
Load:  1 ng per lane.
Primary antibody:  none.
Secondary antibody:  none.
Predicted/Observed size:   25.5 kDa for Glutathione-S-Transferase monomers. 
Other band(s): none.
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Synonyms: GST, Glutathione-S-Transferase

Glutathione-S-Transferase (GST) Control Protein Properties

Description
Glutathione-S-Transferase (GST) Control Protein - 000-001-200
Application
Other Dilution: User Optimized
Epitope Tag Type
GST
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Label
Concentration
0.99 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Glutathione-S-Transferase (GST) Control Protein Description

Background
Glutathione-S-Transferase (GST) Control Protein will bind to glutathione affinity resin and will be detected by ant-GST antibody. Affinity tags are appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Glutathione-S-Transferase (GST) Control Protein is suitable for use as a control in affinity purificaiton and in western blot immunoassays. Optimal concentration should be determined by the researcher.
Purity/Specifity
Glutathione-S-Transferase (GST) Control Protein was purified by chromatography from E.coli preparations.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-GST antibody.  Analysis by SDS-PAGE resulted in a pattern consistent with purified GST and an estimated purity greater than 95%.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
200-101-200

Product Type
GFP and Epitopes;
Applications
FLISA, WB, IF
Catalog Number
200-102-200

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry