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Proteins and Peptides  >  Control Peptides
 

AKT Control Peptide

Code

000-000-401
Size

50 µg
Price

$90.00
Availability

Ships next business day.
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Shipping:
$85.00 to United States
Handling:
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Synonyms:

AKT Control Peptide Properties

Description
AKT CONTROL PEPTIDE - 000-000-401
Species of Origin
Human
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
PSC Type
Synthetic Peptide
PSC Format
Peptide
Label
Concentration
1.0 mg/mL by dry weight
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
Download CofA
Lot#  

AKT Control Peptide Description

Background
Non-specific binding of an antibody to proteins other than the antigen can sometimes occur. This is usually more common with polyclonal antibodies, but can also occur with monoclonals as well. To determine which band or staining is specific, the AKT immunizing peptide blocking experiment can be performed. Prior to the staining protocol, the anti-AKT antibody is neutralized (incubated with an excess of peptide that corresponds to the epitope recognized by the antibody). The AKT antibody that is bound to the blocking peptide is no longer available to bind to the epitope present in the protein on the Western blot or in the cell. The neutralized antibody is then used side-by-side with the antibody alone, and the results are compared. By comparing the staining from the blocked antibody versus the antibody alone, you can see which staining is specific: this staining will be absent from the Western blot or immunostaining performed with the neutralized antibody.
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Akt blocking peptide should be used at 1.0 µg per 1.0 µl of antiserum in per assay.
Purity/Specifity
Greater than 95% specific peptide
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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AMCA Conjugates
Antigen Retrieval Methods
Azide Removal by Dialysis
Beta Galactosidase Conjugates
Biotin Binding Assay (HABA)
Biotin Conjugates
Cell Lysates Protocol
CyDye™ Conjugates
DyLight™ Antibody Conjugate Properties
DyLight™ Antibody Spectra
ELISA
ELISA to Quantify Collagen
Extended Conjugate Information Sheet
Fab Fragment Monovalent Antibodies
FACS / Flow Cytometry Analysis
Fluorescein Conjugates
Gel Shift Assay
General Antibody Information
Histone Immunoblotting
Immunoblotting
Immunocytochemistry
Immunofluorescence Microscopy
Immunofluorescence Microscopy - Assay Formats
Immunofluorescence Microscopy - ATTO-TEC
Immunofluorescence Microscopy - CyDye®
Immunofluorescence Microscopy - DyLight™
Immunofluorescence Microscopy - Fluorescent Labels
Immunofluorescence Microscopy - IRDye®
Immunofluorescence Microscopy - long
Immunofluorescence Microscopy - Microscope Types
Immunofluorescence Microscopy - Other Conjugates
Immunofluorescence Microscopy Protocol
Immunohistochemistry
Immunoprecipitation
Nuclear/Cytoplasmic Extract Isolation
Peroxidase & Alkaline Phosphatase
Phosphorylated State Proteins
Phosphorylated State Proteins - AKTs
Phosphorylated State Proteins - Long
Phosphorylated State Proteins - Pathways
Phosphorylated State Proteins - Phospho Specific
Phosphorylated State Proteins - Tailored Phosphorylated Target
Phosphorylated State Proteins - Western Blot
Phycoerythrin Conjugates
Protein A / Protein G Binding Reactivities
Recommended Dilutions
Rhodamine & Texas Red™
SDS-PAGE
Staining Paraffin Sections by PAP Procedure
USDA & Material Safety Notice
Western Blot - Buffers
Western Blot - Long
Western Blot - Preassembled Kits
Western Blot - Primary Antibody Reagents
Western Blot - Secondary Antibody Reagents
Western Blot - Substrates
Western Blot - Types of Blot Formats
Western Blot (Protein Immunoblot)
Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry