Bio-Rad has collaborated with Rockland Immunochemicals to develop an enhanced multiplex fluorescent western blotting protocol. The assays produce high resolution immunoblots on PVDF membrane, providing increased visibility and profiling of low abundance proteins.
Rockland DyLight™ fluorochrome conjugated secondary antibodies are used to probe the membrane and the Molecular Imager® VersaDoc™ MP 4000 and the ChemiDoc™ XRS+ imaging system developed by Bio-Rad Laboratories, provides clear, accurate results. Several targets can be detected and imaged simultaneously, resulting in blots that show enhanced detection of distinct target bands labeled by a unique identifying color. Simultaneous detection helps cut time, while ensuring the target epitopes are still easily identified.
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