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Host Cell Protein (HCP) Antibody Generation


Host Cell Protein Antibody Generation Overview

 

Biopharmaceuticals require products to be free of host cell protein (HCP) contaminants.  These HCPs may be left behind during the purification process from the expression hosts, such as E. coli, insect, or mammalian cells.   To investigate the presence of residual contamination during the bioprocessing and the final biopharmaceutical product, development of custom polyclonal antibody reagents with maximum coverage against native HCP extracts is required. Please see US FDA Guidelines for Bioanalytical Validation.

 
  
Rockland provides multiple customizable options for HCP antibody development.  These options allow flexibility to the researcher to develop early stage through late stage HCP detection reagents.

 

 

 

  • Related Bioprocessing and Host Cell Protein Reagents

Bioprocessing and Host Cell Protein Reagents

Bioprocessing Related Products

Catalog Number

Applications Size
       
Anti-Bovine Serum Albumin (Rabbit) Antibody

201-4133-0025

ELISA, WB, IHC 25 uL
Anti-Bovine Serum Albumin (Mouse) Monoclonal Antibody (BSA)

201-301-033

ELISA, WB, IHC, IP 500 uL
Anti-Streptavidin (Rabbit) Antibody

200-401-095

ELISA, WB, IHC 100 uL
Anti-Biotin (Mouse) Monoclonal Antibody

200-301-098

ELISA, WB, IHC, IF 100 uL
Anti-6X His Epitope Tag (Mouse) Monoclonal Antibody

200-301-382

ELISA, WB, IP 100 ug
Antibody For The Detection Of Flag™ Conjugated Proteins (Mouse) Monoclonal Antibody

200-301-383

ELISA, WB, IHC 100 ug
Anti-Protein A (Goat) Antibody

100-1177

ELISA, WB, IHC 2 mL
Anti-Protein G (Rabbit) Antibody

100-401-138

ELISA, WB

2 mL

Anti-Bovine Igg (H&L) (Rabbit) Antibody

201-4102

ELISA, WB, IHC 50 mg
       
Host Cell Protein Related Products

Catalog Number

Applications Size
E.Coli Host Cell Protein Control Protein

000-001-J08

ELISA, WB 50 ug
Anti-E.Coli  Host Cell Protein [Total] (Rabbit) Antibody

100-401-J07

ELISA, WB, IHC 100 uL
Anti-E.Coli  High Molecular Weight Fraction Host Cell Protein Antibody

200-401-J05

ELISA, WB 100 ug
Anti-E.Coli Low Molecular Weight Fraction Host Cell Protein Antibody

200-401-J06

ELISA, WB 100 ug
Anti-E.Coli  Host Cell Protein [High And Low Molecular Weight Combined] Antibody

200-401-M61

ELISA, WB 100 ug
Anti-E.Coli  Host Cell Protein Combined Biotin Conjugated Antibody

200-406-M61

ELISA, WB 100 ug
Chemiluminescent Western Blot Kit For Detection Of E.Coli Host Cell Proteins

KCAJ07

WB 1 Kit

 

 

 

15-02_HCP_Poster_Side_Bar

Download the Multi-assay Functional Antibodies in HCP Detection poster

 

 

HCP Development Poster

Download the Enhanced Standardized HCP poster


 

Host Cell Protein Workflow

 

Producing an HCP Antibody: Workflow