No matter what the application, Rockland antibodies shine through. The western or immuno blot is a work horse assay for most labs, used to demonstrate the presence or absence of important proteins, detect post-translational modifications, diagnosis is disease and more. Robust bands that identify targets can be visualized after probing your blot with Rockland primary antibodies for detection and conjugated secondary reporting antibodies. Protect your experiments with Rockland antibodies. Compromise elsewhere.

Background



The western blot (sometimes called the protein immunoblot) is a widely used method for the detection of specific proteins among a sample of tissue homogenate or extract.  Western blot uses gel electrophoresis (SDS-PAGE) to separate proteins according to the protein molecular weight. The proteins are then transferred from the gel onto a membrane (typically nitrocellulose or PVDF).  The membrane is blocked with a protein blocking buffer to prevent nonspecific binding to the antibodies. The membrane is probed using a primary antibody to detect the protein followed by another probing with a secondary antibody conjugated to a reporter molecule to visualize the target protein.  Primary antibodies conjugated to a reporter do not require secondary antibody visualization.  Wash steps with a detergent containing buffer are typically performed after antibody incubations to remove any non-specific binding of the antibodies. Reagents for western blot are available from Rockland as standalone products, but also as easy to use preassembled kits.


1. Types of Western Blot Formats
2. Primary Antibody Reagents
3. Secondary Reporter Antibody Reagents
4. Substrates
5. Buffers and Blocking Reagents
6. Western Blot Preassembled Kits