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Phosphorylated State Proteins


Phosphorylation

Phosphorylation involves the addition of a phosphate group to a serine (S), threonine (T) or tyrosine (Y) residue of a protein by a kinase and is essential for pathway activation for cellular regulation, signaling and growth. Our portfolio offers a large and diverse set of antibodies that detect phosphorylated targets with great precision for use in western blot, flow cytometry, immunohistochemistry and immunofluorescence microscopy.


Be specific with Rockland antibodies.


Background 

Phospho specific Antibodies  

Pathways Covered at Rockland 

Western Blot, Immunohistochemistry and Immunofluorescence  

AKTs 

Tailored Phosphorylated Target  




phosph background

Background

Protein phosphorylation plays a significant role in a wide range of cellular processes including cell growth and proliferation, metabolism, physiological regulation and signaling. Activated proteins usually trigger a cascade of reactions and interactions which lead to cellular activation, motility, proliferation and metabolic modifications. Complexity grows exponentially when one protein is involved in several pathways and initiates or relays different responses. Therefore, accurately and precisely quantifying and tracking phosphorylated proteins at the cellular level is critical to deciphering and understanding cellular functions. At Rockland, we have developed a vast and diverse portfolio of phospho specific antibodies that allow scientists to track and quantify phospho-proteins using a wide range of techniques such as western blot, flow cytometry, immunohistochemistry and immunofluorescence.

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phosph pathways

Pathways Covered at Rockland

With more than thirty years of experience, Rockland’s portfolio has grown to include an impressive collection of phospho specific antibodies. Our philosophy is to constantly meet new challenges- we continuously expand our collection of phospho specific antibodies for applications in neuroscience, cellular growth and metabolism, cancer research, cell signaling, epigenetics, apoptosis, autophagy and responses to the extra-cellular environment.

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phosph targets

AKTs and Related Targets

The Akt family of serine-threonine kinases consists of three members: Akt-1, Akt-2 and Akt-3. All Akt family members share a common structure with an N-terminal plekstrin domain, a central kinase domain and C-terminal hydrophobic domain. The Akt family members are not redundant and regulate a broad spectrum of cellular functions. To be fully activated, Akt requires phosphorylation at two specific sites: threonine 308 and serine 473. Upon activation, Akt moves from the membrane to the cytoplasm and nucleus where it phosphorylates its target.

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phosph specific

Phospho Specific Antibodies

Rockland offers a wide variety of primary and secondary phospho specific antibodies validated by a variety of immunoassays including western blot protocol, immunohistochemistry and flow cytometry. Antibodies recognize phosphorylated target proteins involved in the apoptotic pathway, the cell cycle, stem cell development, cancer and other pathways of critical interest to life science researchers.

Rockland offers a wide range of monoclonal and polyclonal antibodies specific for activated Akt at positions pT308 and pS473.

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phosph western

Western Blot, Immunohistochemistry and Immunofluorescence

Rockland conjugates phospho specific antibodies to cutting edge fluorochromes (ATTO, DyLight™, CyDyes™ and IRDyes®), peroxidase and biotin. Combined with a broad range of secondary antibodies, our primary conjugated phospho specific monoclonal and polyclonal antibodies are perfect choices for high quality western blot, immunohistochemistry and immunofluorescence microscopy.

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phosph custom

Tailored Phosphorylated Target

Custom phospho specific antibody services includes synthesis of 30 mg of a phospho peptide up to 15 amino acids in length at >90% purity and 20 mg of a non-phospho peptide up to 15 amino acids in length at >90% purity. Additional amino acids, acetylation or amidylation are possible. The peptide synthesis will include a Certificate of Analysis with HPLC trace and mass spectroscopy analysis. Peptides will be KLH and BSA conjugated for immunization and testing. All custom antibody samples will be assayed by ELISA. Your custom phospho specific antibody will then be affinity purified followed by non-specific cross adsorption.

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