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        6X His Affinity Tag Antibody


6X HIS Affinity Tag Antibody Overview



Recombinant proteins are frequently produced with a poly-Histidine epitope tag to allow for detection and affinity purification.  These “HIS-tagged” recombinant proteins may be produced in many different host species including E. coli, insect, yeast, or mammalian cells.  The 6X His tag is typically located at either the amino or carboxyl terminus (also called N-terminal HIS tag or C-terminal HIS tag).  

Rockland’s Anti-6X Histidine affinity tag antibodies are highly specific to polyhistidine epitope tags used when producing recombinant protein.  Rockland’s anti-6X HIS antibody can bind to the typical 6X HIS, but can also bind to less frequently used larger 8X or 10X histidine epitopes.  These antibodies are excellent for detection of 6X HIS tagged recombinant proteins.  

Featured Products: 

 

 

600-401-382

 

200-301-382-6x-HIS

 

600-430-382-6XHIS

Anti-6X HIS EPITOPE TAG

(RABBIT) Antibody 

 

Anti-6X HIS EPITOPE TAG
(MOUSE) Monoclonal Antibody

 

Anti-6X HIS EPITOPE TAG
(RABBIT) Antibody IRDye® 700DX Conjugated 

 

View all 6xHis

  

 


 

 

The Rockland Control Peptide Advantage


Rockland manufactures and validates anti-6X histidine antibodies against multiple recombinant proteins tagged at the amino or carboxyl terminus.  The antibodies are characterized for robust specific activity.  Rockland also produces anti-6X HIS antibodies as peroxidase enzyme or dye reporter conjugates, providing the greatest flexibility to the end user in the design of their assay.  Rigorous quality control testing ensures that the final product meets or exceeds Rockland’s standards for robust performance in your assays.

 


 

6X-HIS Epitope Antibodies

 

Product Name

Catalog #

Applications

Size

Anti-6X HIS EPITOPE TAG Antibody

600-401-382

WB, IP, ELISA

100 µg

Anti-6X HIS EPITOPE TAG Antibody Peroxidase Conjugated

600-403-382 WB, IP, ELISA

100 µg

Anti-6X HIS EPITOPE TAG Antibody IRDye® 700DX Conjugated

600-430-382 WB, IP, ELISA 100 µg

Anti-6X HIS EPITOPE TAG Monoclonal Antibody

200-301-382 WB, IP, ELISA

100 µg

 Anti-6X HIS EPITOPE TAG Monoclonal Antibody Biotin Conjugated 200-306-382 WB, IP, ELISA 100 µg

Anti-6X HIS EPITOPE TAG Monoclonal Antibody IRDye® 800 Conjugated

200-332-382

WB, IP, ELISA

100 µg

 

 

6X HIS antibodies can be used but are not limited to the following assays:


Western blot
Immunoprecipitation
• Dot blot
ELISA

See Rockland’s other epitope tag antibodies such as anti-FLAG and anti-GST.

Rockland also offers cell lysates and extracts suitable for use as controls for HIS tag detection in control experiments and Rockland offers custom molecular biology services for the production or recombinant proteins contain HIS tag.

 

 

New Lab Set Up

 
 

 

  

Rockland'sTrueBlot® product line enables researchers to generate publication-quality IP western blot data. Simply substitute your conventional HRP blotting reagent with Goat TrueBlot®, Mouse TrueBlot®, Rabbit TrueBlot® or Sheep TrueBlot®.

 

TrueBlot® is a horseradish peroxidase conjugated immunoblotting detection reagent, which enables unhindered detection of blotted target bands. This results in unparalleled clarity in results and high quality imaging suitable for reproduction.