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2D Gel

2D Analysis


Protein contaminant detection can be visualized on 1D electrophoresis with western blotting. However, the immunogenicity of individual HCPs can vary widely and highly immunogenic proteins can disguise or “mask” the effects of other, similar size proteins. The implementation of 2D SDS-PAGE assessment, by which complex protein mixtures are separated according to isoelectric point and molecular weight followed by western blotting, can deliver a more complete insight into the capability of an anti-HCP polyclonal antibody with respect to immuno-coverage of protein components of the host cell lysate.  Rockland can customize to meet your 2D  Analysis with both a Midi (8 X 13 cm) and Large Gel Format (17 X17 cm)


2D Gel



Click 1D Analysis



Related Links:


Host Cell Protein

Standard HCP Reagent Development

HCP Assay Development

Cascade Antibody Generation

1D Analysis